5-Keto-d-fructose

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Preparation of enzymes required for enzymatic quantification of 5-keto-D-gluconate and 2-keto-D-gluconate.

For easy measurement of 5-keto D-gluconate (5KGA) and 2-keto D-gluconate (2KGA), two enzymes, 5KGA reductase (5KGR) and 2KGA reductase (2KGR) are useful. The gene for 5KGR has been reported, and a corresponding gene was found in the genome of Gluconobacter oxydans 621H and was identified as GOX2187. On the other hand, the gene for 2KGR was identified in this study as GOX0417 from the N-terminal...

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Sucrose/D-Glucose/ D-Fructose

1. Bottle 1 with approx. 0.5 g lyophilizate, consisting of: citrate buffer, pH approx. 4.6; β-fructosidase, approx. 720 U 2. Bottle 2 with approx. 7.2 g powder mixture, consisting of: triethanolamine buffer, pH approx. 7.6; NADP, approx. 110 mg; ATP, approx. 260 mg; magnesium sulfate 3. Bottle 3 with approx. 1.1 ml suspension, consisting of: hexokinase, approx. 320 U; glucose-6-phosphate dehydr...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1970

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)63237-5